For some types of TEM installed with multiple EDX detectors, the X-ray signals excited would not entirely be blocked by the bulk body of k-kit. We usually can get it a clear signal from K-kit, no need to turn any of body rotation or tilting.

The EDX signal of gold could be detected directly, no need to make any tilt at the K-kit body. The gold nanoparticles were formed from the reduction process of AuCl4 solution in K-kit, and we used FEI Talos TEM, which with four EDX dectors around the specimen area of the TEM column, in this test.

EDX detectable areas of k-kit in different kinds of TEM equipment

All k-kits were loaded with AuCl3 solution. Au nanoparticles would be separated out at the moment, when the liquid sample AuCl3 in k-kit under an electron beam irradiation during TEM observation. By detecting the Au signals from a K-kit with different orientations and tilt angles in TEM, one may clearly define how to make a K-kit achievable for EDX analysis accordingly.

Example I   TEM: Hitachi 7700 EDX: Single Detector  Liquid Sample: AuCl3 Tilt Angle: 0°, 10°, 20° Measured Area: Center

Example II   TEM: Hitachi 7700 EDX: Single Detector  Liquid Sample: AgNA Sample Prep.: Wet Mode Tilt Angle: 20° Measured Area: Left Site	(The location to be measured)